Translational Analysis Core
This core provides VDDRC investigators with the opportunity to move nimbly from investigations utilizing non-invasive molecular imaging and histology, often in tandem, in tractable transgenic models of digestive diseases to digitization and state-of-the-art high throughput analysis of morphology-based findings.
Core director: M. Kay Washington, MD, PhD
This core includes three components:
- Digital Histomorphology Service
- Tissue Morphology Service
Digital Histomorphology Service
Co-Directors: James R. Goldenring, MD, PhD and Tae Hyun Hwang, PhD
Specific Aim: To provide VDDRC researchers with cutting-edge digital histomorphology services
Services:
- To provide high-resolution digital imaging of whole slides and TMAs in brightfield and fluorescence
- To facilitate the use of multiplexed fluorescence staining of histological specimens
- To facilitate the use of quantitative methods for analysis of digital histology brightfield and fluorescence imaging
- To provide VDDRC researchers with access to cutting-edge application of artificial intelligence in morphological analyses as well as integration with other spatial analysis modalities by other VDDRC supported cores.
Tissue Morphology Service
Director: M. Blanca Piazuelo, MD
Associate Director: M. Kay Washington, MD, PhD
Specific Aim: To provide VDDRC researchers with high-priority access to state-of-the-art immunohistochemistry (IHC), expert morphologic interpretation, and imaging-histology co-registration services for human and animal tissues.
Services:
- To provide expertise in developing, performing, and evaluating IHC studies for VDDRC members
- To provide expertise in evaluation of histopathology of animal models that closely recapitulate gastrointestinal diseases and direct correlation with human disease and organoid morphology
- To provide access to and morphologic assessment of human GI tissue samples, including custom tissue microarray design