Flow Cytometry Core


The Flow Cytometry Core offers analysis of cell phenotype and properties; digestive disease specific training, troubleshooting, support and optimization for Vanderbilt Digestive Disease Research Center members.

Core Co-Directors: Scott W. Hiebert, PhD and Eric Skaar, PhD, MPH   
Core Manager: David K. Flaherty    

    Core Aims

    • Provide access to state-of-the art equipment for
      • Analysis of cell phenotype and properties 
      • Sorting cells or sub-cellular elements 
    • Furnish professional guidance in experimental design and data interpretation 
    • Assist users in sample preparation of cells, sub-cellular preparations, and secreted factors for optimal analysis 
    • Train users, on a continuing basis, in development of protocols and basic and advanced use of the flow cytometry equipment and software 
    • Assist users with grant and publication material, data analysis, and figure preparation 
    • Provide digestive disease specific training, troubleshooting, support and optimization to VDDRC members via the Flow Cytometry Core 

    Core Services

    • Training, acquisition and analysis on our state-of-the-art cytometers for experiments that include up to 17 different fluorochromes simultaneously 
    • High-speed sorting with up to 15 different fluorochromes simultaneously with the ability to sort and or clone into any plate or dish 
    • Mass Cytometry with the potential to measure 30-100 antigens simultaneously 
    • Three levels of biocontainment to address the needs of every VDDRC member 
    • Imaging Cytometry 
    • Expert consultation and troubleshooting and experimental design guidance