My project focuses on identifying the role of oxidative stress pathways in the development and maintenance of functional beta cell mass. One aspect of my project examines the consequence of changes to antioxidant stress in the presence of metformin, a common Type 2 Diabetes therapeutic, on NHP offspring islet function during fetal development and as they approach adolescence. The other aspect of my project builds on previously published results in a mouse model of T2D where antioxidant stress in adult islets is ameliorated by treatment with an inhibitor of the GPCR, EP3, whose ligand, PGE2 is a metabolite of arachidonic acid. EP3 antagonism was also demonstrated to increase the expression of the GLP-1R in beta cells, identifying the interplay between these pathways may provide insight into how combined T2D therapeutics can be utilized to improve human health.