Phenotyping & Pathophysiology Core

The Phenotyping and Pathophysiology Core is designed to address the need for effective phenotyping of renal function in mouse models of selective gene overexpression or deletion. 

  • Designed to provide experimental mouse models of acute or progressive renal injury to assess potential therapeutic interventions.

  • Helps investigators who may have developed genetic mouse models or potential therapeutic interventions, but do not have the expertise and/or infrastructure required to design and carry out appropriate kidney phenotyping studies or studies investigating mouse models of kidney injury.

The Phenotyping and Pathophysiology Core has five distinct subcores that will offer and/or develop methodologies to define the pathogenesis of kidney disease and design therapeutic interventions.

  • The Phenotyping subcore will provide a battery of well-established invasive and non-invasive tests of kidney function.
  • The Injury Model subcore will provide investigators cost-effective testing of potential pharmaceutical or immunologic therapy strategies in appropriate mouse models of acute or progressive kidney injury. In addition, this subcore will provide murine models for screening of potential kidney side effects of drugs. This subcore provides a wide variety of experimental models of mouse kidney injury that offer consistent and reproducible results. Some of the mouse models available have been developed by the Center investigators.
  • The Metabolism and Bioenergetics subcore will offer direct measurements of kidney tissue partial oxygen consumption and will develop methodology for the measurements of whole kidney oxygen consumption based on kidney blood flow and measurements of arterial and renal venous partial oxygen. Furthermore, this subcore will provide expertise in metabolic flux analysis, glucose uptake, and quantification of various metabolites in kidney tissues using mass spectrometry methods.
  • The In Vivo Mouse Kidney Imaging subcore will develop and establish non-invasive imaging techniques for the assessments of mouse renal diseases. This subcore will help investigators to execute high throughput, quantitative, and non-invasive imaging studies in mouse models of renal disease.
  • The CRISPR/cas9 subcore will provide guidance for the design, construction, and generation of a CRISPR mouse

The Phenotyping and Pathophysiology Core provides a broad range of services that aid researchers interested in utilizing murine models for kidney-related research. The structure of this Core is unique in that it performs a variety of kidney functions, non-invasive quantitative kidney images analysis, and experimental platforms for the testing of drugs and interventions. 

Most importantly, this Core performs all these services in-house and directly oversees these studies. We have been very successful in providing these services not only to the local scientific community interested in kidney research, but also serving as a national and international resource.

We not only provide innovative services, but also plan to continue to develop, test and implement new methodologies that can be utilized by both the local Biomedical Research Base, as well as interested national and international researchers, to advance our knowledge about the mechanisms of kidney disease, and to develop and test potential therapies.

Core Assays and Service

Core Leadership

Ming-Zhi Zhang, MD

Associate Professor of Medicine, Division of Nephrology and Hyptertension


Ambra Pozzi, PhD

Professor of Medicine, Division of Nephrology and Hypertension
Co-director, Vanderbilt O'Brien Kidney Center


Eric Delpire, PhD

Professor of Anesthesiology, Vanderbilt University School of Medicine


Volker H. Haase, MD

Professor of Medicine,
Division of Nephrology and Hypertension


Takamune Takahashi, MD, PhD

Professor of Medicine,
Division of Nephrology and Hypertension


Please contact Core leadership for pricing.

For more information and questions, please email Jennifer Rieke, Program Manager, or call (615) 343-5400.