Workshops


As part of its education and outreach program, the Vanderbilt O'Brien Kidney Center (VOKC) organizes two, intensive, five-day, in-person, hand-on and practical mouse kidney research workshops each year. 

These are designed for laboratory staff, trainees and/or faculty interested in learning more about the practical challenges of mouse kidney research, developing new skills and/or mastering techniques to transfer back to their own institutions. By the end of each course attendees will be sufficiently versed in these techniques to be able to set them up at their own institutions.

Mouse Kidney Injury Workshop

Five-day, hands-on, supervised training workshop focused on kidney injury models, surgical techniques, tissue and body fluid collections, and renal function tests in mice

This is a hands-on, practical training course designed to teach surgical and phenotyping techniques for commonly used mouse renal injury models, including ischemia/reperfusion (IR), UUO, 5/6 nephrectomy and the cecal ligation and puncture model of AKI.

A panel of experts has been assembled to teach basic animal handling, drug administration, tissue and blood sampling techniques (Day 1), tissue harvesting and analyses (Day 2) and mouse surgery technique (Days 3 to 5) with lectures, training videos, practical demonstrations and hands-on practical training techniques.

By completion, participants will be sufficiently familiar with practical procedure to be able to set up the models in their own labs.

There is no other practical workshop like this anywhere!

Reasons to attend:

  • You plan to set up models of kidney injury in mice but have no practical expertise locally
  • You have new personnel joining your lab who need to be trained to study mouse kidney injury models, but you do not have personnel or time to do this yourself

Contact the Mouse Kidney Injury Workshop organizer:

Mark de Caestecker, MBBS, PhD

Professor of Medicine, Division of Nephrology & Hypertension

Email​​​​​​​

For more information and questions, please email Karen Thompson, Program Manager, or call (615) 343-5400. 


Renal Translational Pathology Workshop

Three-day intensive workshop that focuses on mouse kidney pathology, including training in quantitative and qualitative analyses of different renal pathologies and stains

The Vanderbilt O'Brien Kidney Center (VOKC) is organizing a 2-day, comparative renal pathology workshop with didactic and hands-on training in qualitative and quantitative renal pathology February 23rd and 24th 2023 at Vanderbilt University Medical Center in Nashville. Drs. Agnes Fogo and Haichun Yang from the Division of Renal Pathology at Vanderbilt University Medical Center have developed a virtual slide-based workshop to train pre-clinical investigators on the fundamentals of human and experimental renal pathology, focusing on similarities and differences between human disease and mouse models used to study these human diseases. They will be assisted by Dr. Charles Jennette from the Department of Pathology at the University of North Carolina, Chapel Hill, Dr. Mark de Caestecker and Dr. Raymond Harris from the Nephrology Division at Vanderbilt University Medical Center, who will be providing additional content expertise. The focus of the workshop will be to train investigators to understand the relevance of experimental models of human disease and to understand how to interpret and quantify histological findings using these models so they can bring this expertise back to their own institutions.

Four topic areas (diabetic nephropathy; focal segmental glomerular sclerosis; acute kidney injury; and crescentic kidney disease) will be covered, with the goal to train investigators to recognize:

  • patterns of renal injury
  • similarities and differences between human and mouse models of kidney disease, as well as species and strain differences in renal pathology seen using different experimental models; and
  • to be able to quantify cell specific pathologic changes using the appropriate histological staining. The format of the workshop will include lectures covering basic background about the human diseases, the mouse models used, the quantitative methods used to evaluate them, alternating with hands-on interactive training using computer-based virtual slides to illustrate the pathologies being discussed. Each student will be required to bring their own laptop.

The format of the workshop will include lectures covering basic background about the human diseases, the mouse models used, the quantitative methods used to evaluate them, alternating with hands-on interactive training using computer-based virtual slides to illustrate the pathologies being discussed.

Reasons to attend:

  • You study mouse renal disease models, but have not been trained how to evaluate different renal pathologies, and do not have expertise at your institution to help read and quantify you tissue sections
  • You have new personnel joining your lab who need to be brough up to speed to study mouse kidney injury models
  • You know a little but want to know more about different mouse renal pathologies and their relationship with the human diseases they are modeling

Each student will be required to bring their own laptop.

* Each attendee will have one computer loaded with human and mouse virtual slides and software (image J, Aperio Image Scope). Background of individual human disease or mouse model and working sheet will also be provided.


Four topic areas will be covered over the 2.5 days.

On day one, attendees will receive an overview, samples preparation and staining, and use of Aperio Image Scope software. In the afternoon, programming will focus on diabetic kidney disease-human pathology, mouse models, mouse histology and quantification, and practice mouse histology.

Programming for days two and three will include FSGS, immune-mediated glomerular injury, and AKI and AKI>CKD transition. There will be the option in the afternoon of the third day to go over questions that come up over the course of the workshop, and to practice quantification of different mouse kidney pathologies.

A detailed overview of the program is provided below.

Course application is open now through December 31, 2022.

A maximum of 20 attendees will be selected based on their background experience and needs. Those that are not selected for the first year will be shortlisted for the following year, if required. To apply, please complete the following application on REDCap and email the program manager.


STEP 1 - COMPLETE APPLICATION:


STEP 2 - CONTACT PROGRAM MANAGER:

Jennifer Rieke
Program Manager
Division of Nephrology and Hypertension
1161 21st Ave South, MCN S-3305
Nashville, TN 37232
Phone: 931-249-4010
Fax: 615-343-2675
Email: Jennifer.l.rieke@vumc.org

Day 1

TimeAgenda Item
8:00 - 8:45amAKI-human pathology (Paisit Paueksakon)
8:45 - 9:30am

AKI and AKI-CKD models in mice (Mark de Caestecker)

9:30 - 10:15am

Tubular injury & interstitial fibrosis in AKI and AKI>CKD models (Yang)

10:15 - 10:30am

Break

10:30 - 12:00pm

Practice for semi-quantification-acute tubular injury score (PAS) and interstitial fibrosis (Sirius red and Col 1) (Agnes Fogo and Haichun Yang)

12:00 - 12:30pm

Lunch

12:30 - 1:15pm

Crescentic renal disease-human pathology (Charles Jennette)

1:15 - 2:00pm

Crescentic renal disease models in mice (Charles Jennette)

2:00 - 2:45pm

Glomerular crescent and necrosis quantification in mouse Crescentic model (Haichun Yang)

2:45 - 3:00pm

Break

3:00 - 4:15pm

Practice for quantification - glomerular crescents and glomerular necrosis (PAS) (Agnes Fogo and Haichun Yang)

3:00 - 4:15pmFeedback and closing remarks


Day 2

TimeAgenda Item
8:00 - 8:45amAKI-human pathology (Paisit Paueksakon)
8:45 - 9:30am

AKI and AKI-CKD models in mice (Mark de Caestecker)

9:30 - 10:15am

Tubular injury & interstitial fibrosis in AKI and AKI>CKD models (Yang)

10:15 - 10:30am

Break

10:30 - 12:00pm

Practice for semi-quantification-acute tubular injury score (PAS) and interstitial fibrosis (Sirius red and Col 1) (Agnes Fogo and Haichun Yang)

12:00 - 12:30pm

<Lunch

12:30 - 1:15pm

Crescentic renal disease-human pathology (Charles Jennette)

1:15 - 2:00pm

Crescentic renal disease models in mice (Charles Jennette)

2:00 - 2:45pm

Glomerular crescent and necrosis quantification in mouse Crescentic model (Haichun Yang)

2:45 - 3:00pm

Break

3:00 - 4:15pm

Practice for quantification - glomerular crescents and glomerular necrosis (PAS) (Agnes Fogo and Haichun Yang)

3:00 - 4:15pmFeedback and closing remarks


* Each attendee will need to bring their own computer and preload with virtual slides and digital image software provided via links before the meeting. Background key papers of individual human diseases and mouse models, and work sheets for scoring will also be provided.

Contact the Renal Pathology Workshop organizers:

Mark de Caestecker, MBBS, PhD

Professor of Medicine, Division of Nephrology & Hypertension

Email

Agnes Fogo, MD

Professor of Pathology, Microbiology, and Immunology


Email

For more information and questions, please email Jennifer Rieke, Program Manager, or call (615) 931-249-4010.