Services

Proteomics: Protein Analysis by qPCR and Sequencing

• Next-Generation Sequencing (NGS) based Olink Proteomics: Detect 5,432 protein levels across 2,064 samples simultaneously in one week. This workflow utilizes Olink Explore HT kits and requires only 3 µl of sample for the entire analysis. Results provide relative protein expression data.
• Detect 1,032 inflammatory proteins with Olink Reveal panel.
• qPCR-Based Proteomics: Choose from 14 human qPCR panels and 1 mouse qPCR panel (each targeting 92 unique proteins). Each panel run accommodates 96 total targets and 88 samples. Results are ready in 2 days using just 1 µl of sample.
• Accepted Sample Types: Plasma, serum, tissue, cell lysates, and various body fluids.
• Inquiries: For unlisted sample types or project consulting, contact us at htbiomarkercore@vumc.org

High-Throughput Exosome Isolation, Exosome RNA Isolation & Library Services

• Isolation & Library Prep: We isolate up to 192 exosome samples per day from plasma, serum, or other biofluids with size exclusive chromatography.
• Downstream Processing: Automated workflows feed directly into high-throughput RNA isolation followed by whole transcriptome and/or small RNA library preparation.

OpenArray Gene Expression and Infectious Disease Pathogens Detection

• Our OpenArray Gene Expression and Infectious Disease Pathogens Detection services deliver high throughput, nanofluidic qPCR screening for hundreds of targets simultaneously.
• Scale your research with our OpenArray platform, offering ultra-high-throughput profiling for both gene expression analysis and rapid infectious disease pathogen detection.

Single-Cell Gene Expression Analysis

• High-Throughput RT-qPCR: Analyze up to 96 live single cells across 96 different gene targets.
• User Delivery: Simply bring your captured single cells and preferred gene expression assays to our core, and we will perform the high-throughput analysis for you.

miRNA and Gene Expression Profiling

• Scale & Speed: Profile hundreds to thousands of miRNAs or gene expression targets across hundreds to thousands of samples rapidly and affordably.
• Data Quality: We utilize gold-standard qPCR instrumentation to ensure highly sensitive, reproducible data.
• Technical Advantage: Unlike standard RNA-seq, high-abundance miRNAs or genes do not saturate or bias our qPCR analysis.

SNP Genotyping

• Throughput: Screen 96 samples against 96 SNPs in under 3 hours.
• Assay Compatibility: Fully compatible with standard TaqMan SNP assays and rhAmp SNP Genotyping Assays from IDT DNA Technologies.
• Custom Design: Users can submit custom targets for the SNPtype assay design service via Standard BioTools.
• Inquiries: Contact us at htbiomarkercore@vumc.org for assay design guidance and pricing details.